Cytochrome P - 450 - dependent I 4 a - sterol demethylase of Candida albicans and its interaction with mole antifungals
نویسنده
چکیده
14. 15. Canonica, I+ Fiecchi, A., Galli Kienle, M., Scala, A., Galli, G., Grossi Paoletti, E. & Paoletti, R. (1968) J. Am. Chem. SOC. 90,3597-3598 Yoshida. Y. & Aoyama. Y. (1900) Chirality, 2, 10-15 Yoshida. Y.. Aoyama, Y.. Takano. H. & Kato, T. (1980) Hiochem. Hiophys. Kes. Commun. 137. 5 13-5 10 16. Anderson, N. H., Branch, S. K., Thomas Loefier, R. S., Mann, H. E., Nowell, 1. W. & Walker, 1'. E. (1 984) I'estic. Sci. 15, 3 10-3 10
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Purification, reconstitution, and inhibition of cytochrome P-450 sterol delta22-desaturase from the pathogenic fungus Candida glabrata.
Sterol delta22-desaturase has been purified from a strain of Candida glabrata with a disruption in the gene encoding sterol 14alpha-demethylase (cytochrome P-45051; CYP51). The purified cytochrome P-450 exhibited sterol delta22-desaturase activity in a reconstituted system with NADPH-cytochrome P-450 reductase in dilaurylphosphatidylcholine, with the enzyme kinetic studies revealing a Km for er...
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